The goal of this research was to explore potential changes in the blood levels of cholesterol, high-density lipoprotein cholesterol, calcium, and zinc among people diagnosed with breast cancer. This study compared the concentrations of these substances in the sera of patients to those of healthy subjects. The findings revealed significant differences, with patients exhibiting changes indicative of structural negative alterations. The observed variations are statistically significant (p < 0.05), underscoring the need for ongoing monitoring and the development of suitable interventions. Additionally, our findings suggested a possible association between elevated calcium levels in non-cancerous breast tissue and a heightened risk of subsequently developing breast cancer.