This study is investigated on the barks of a specific species of Pouteria genus known as Planchonella obovata (PO) from the family Sapotaceae to identify antioxidant, cytotoxic and analgesic activity. Several studies have been explored that many species of have showed medicinal properties like antioxidant, anti-inflammatory, anticancer, antimicrobial property and for this reason species of Pouteria genus are used for treating purpose from many years. Barks of Planchonella obovata collected and extracted with ethanol solvent by soxhlet extraction process. Antioxidant studies of ethanolic extract of barks of Planchonella obovata as DPPH free radical scavenging assay found that IC50 value 1.15µg/ml compare with standard Ascorbic acid 3.338 µg/ml, total phenolic content 145.56±2.55 mg/g, total flavonoid content is 8.75±9.922mg/g, total Antioxidant found 250±6.614 mg/g and Reducing power capacity 46.4% assuming standard as 100%. In cytotoxicity study LC50 value 3318.47µg/ml compare to standard vincristine sulfate (LC50 value 2.313µg/ml). In Hot Plate test dose 500 and 700 (mg/kg) showed the maximum % of elongation respectively 110.96% at 120 min and 150.16% at 60 min compare to standard (Diclofenac Sodium BP-100mg/kg) 55.15% at 120 min which much lower than the extract of Planchonella obovata. The acetic acid induced writhing test at dose 300,500,700 mg/kg showed significant (p<0.001) %of inhibition 65.75%, 76.71% and 84.93% respectively where the standard has 56.16% (p<0.05).In The ethanolic extracts of Planchonella obovata barks found mild antioxidant activity with highly significant DPPH scavenging capacity. Insignificant cytotoxic activity found and showed good Analgesic activity.