Staphylococcus aureus isolates developing transparent zones around colonies were considered to have β- or α-hemolytic activities on blood agar plates. The results show 17 (100%) isolate of S. aureus are positive result for production of heamolysin enzyme. Klebsiella pneumoniae on blood agar medium, bacterial isolates were large, mucoid, white to grey and Non-heamolytic colonies. The result shows that non-heamolytic to blood (γ-hemolytic), all 12 isolates are negative for heamolysis. All K. pneumoniae isolates were found to produce protease enzyme. The formation of clear zone 12 mm in the medium surrounding the well indicated positive protease activity. S. aureus isolates when cultured on trypticase soy agar plates supplemented with gelatine shows to be small, white, circular, slightly convex, entire, smooth, glistening colonies. Compared with K. pneumoniae which shows to be big, white, mucoid, singly in pairs or short chains. Illustrated that all S. aureus isolates are positive gelatinases production, In this study 17 isolate of S. aureus and 12 isolate of K. pneumoniae were collected, siderophore production assay is used to detect the ability of both S. aureus and K. pneumoniae isolates to produce this virulence factor. Siderophore production has been shown more frequent in Klebsiella isolates than in Staphylococcus. S. aureus can produce siderophore appear pink on minimal 9 agar plates. K. pneumoniae can produce siderophore appeared pink on minimal 9 agar plates. Cup assay or agar-well diffusion method has been used to detect of 17 S. aureus, 12 K. pneumoniae for bacteriocin production by using brain heart infusion agar plate supplemented with 5% glecerol as growing of producing isolates, the result shows that 5 isolates (K1, K9, K10, K11, K12) of K. pneumoniae have the ability to produce bacteriocin that had its effect on some isolates of bacteria (E.coli and S. aureus