srjalsSRJALSSRJALS2788-9386https://doi.org/10.47310/srjals.2022.v02i02.027Genetic and Immunological Study of Beta Thalassemia Patients in Baghdad Province / IraqRawaaNajimAlkhamessiCollege of Science, Mustansiriyah University, Baghdad, IraqBy assessing the complement system serum levels (C3, C4), this investigation sought to investigate the humeral immunity and genetic variation of β-globin genes in Beta Thalassemia Major (BTM) patients at the thalassemia centre at Al-Karama hospital. Eighty BTM patients, aged 6 to 25, have been participated in the investigation. The patients were divided into three groups based on how old they were. Most people were between the ages of 11 and 20 (47.5%). While there were 29:80 (36.25%) patients under 10 years old and 13:80 (16.25%) patients over 20 years old.As a result, A PCR examination of the β-globin gene's genetic variation found no difference in the PCR product's band size. Sequencing findings indicated nucleotide alterations, such as (A) to (C) at location (250), (T) to (C) at location (426), (C) to (A) at location (623), (G) to (A) at location (630) and (T) to (A) at location (724). Moreover, β-thalassemia patients' β-globin genes have three transversion mutations and two transition mutations. Serum C3 levels were found to be lower in BTM patients aged 11-20 years and beyond 20 years compared to those aged 10 years or less, however there was no discernible variation in serum C4 levels across age groups of BTM patients.