srjms

SRJMS

2788-9483

https://doi.org/10.47310/srjms.2023.v03i02.005

Molecular Study to Detect Some Virulence Factors in Methicillin – Resistance Staphlococcus Aureus Isolated from Eye Infections in Wasit Province

NofelHossin Amhi

Alshammari

HaiderKareem

Jassem

Msc Molecular Biology from Ilam University, Al-Azizia General Hospital, Al-Mursalat, Makkah

Msc Microbiology from Ilam University, Al-Azizia General Hospital, Al-Mursalat, Makkah

Staphylococcus aureus is an opportunist that causes systemic infections and eye infections in the human being body. This organism increases its resistance to many categories of antibiotics all day and turn out to be more resistant. Given this fact, a total of one hundred fifty-four (N =154) samples from eye infections had been collected from patients who had a major complaint of various eye infections: conjunctivitis 51 (33.1%), blepharoconjunctivitis 39 (25.3%), blepharitis 27 (17.5%), dacryocystitis 19 (12.3%) and other external eye infections 18 (11.6%). And attended at to Al-Zharr Hospital and private clinics in Kut city of Wasit province during the period from December 2022 to May 2023. Our results show 63/154 (40.9%) was S. aureus. These isolates were identified according to culture, microscopic examination, biochemical tests and APIstaph system identification kits. The current study show the highest percentage of S. aureus infections was observed in conjunctivitis 23 (36%), followed by blepharoconjunctivitis and the frequency of S. aureus was 15 (24%), then blepharitis 10 (16%), dacryocystitis 8 (13%) While other external eye infections was 7 (8%) in external eye infections. The results showed 37/63(58.73%) of isolated S. aureus were MRSA strain. %), regarding of virulence factor the results showed Eta gene is present in all cases in percent 100%, while other gens appear in different percentages; SeA,94.5 Seb 39.68%, Sec 34.92%, Sed 28.57%, Tst 34.92%, and, Etb 17.46%. The aim of study was to detect. The prevalence of Methicillin–Resistance Staphlococcus aureus isolates among external eye infections.