iarjcmbIARJCMBIARJCMB2789-6005https://doi.org/10.47310/iarjcmb.2024.v0i402.007Study of the Hormonal and Biochemical Variations of Breast Cancer Patients in Kirkuk City, IraqAhmedMaterSailh WasmeYildizHasanTahseenBiochemistry Branch, Kirkuk Medical College, University of Kirkuk, IraqBackground: Breast cancer is the second most prevalent non-skin cancer type (after lung cancer) and the fifth most common cause of cancer death in women, accounting for 10.4% of all cancer incidences. Aim: To study hormonal and biochemical changes pre and post-chemotherapy in breast cancer patients. Materials and Methods: The current investigation, a retrospective observational analysis, was conducted on 249 patients with breast cancer with a mean age of 47.07 years from February 25 to July 28, 2024, at the Oncology Center in Kirkuk, Iraq. The patients were monitored at two distinct intervals, predominantly prior to receiving chemotherapy and post-chemotherapy. CA-15-3 and CA 27.29, Estrogen, Testosterone, Vit D3, S. Creatinine and B. Urea were tested for the patients. Results: An upregulation of CA 15-3 mean concentration level (40.36 U/mL) among breast cancer patients, this was also applicable for CA 27.29 mean level (41.40 U/mL), while these tumor markers downregulated post-chemotherapy. In regards to Testosterone, Estrogen and Vit. D3 mean levels, our study showed great significant results before and after taking chemotherapy. Conclusions: Breast cancer prevalence is high in Kirkuk city. Older ages are more prone to get the disease. Chemotherapy significantly decreases CA 15-3, CA 27.29, Testosterone and Estrogen levels.