iarjcmbIARJCMBIARJCMB2789-6005https://doi.org/10.47310/iarjcmb.2024.v04i01.009Molecular Identification of a New Local Strain of Ureaplasma Urealyticum Isolated from Women Who Use IUDSarahBadriMahmoodAmeerahMahmoodMohammadAl-Khansa Teaching Hospital, Mosul- Iraq, 41003University of Mosul/College of Science/Department of Biology/ Mosul-Iraq, 41003Ureaplasma species are the most prevalent genital Mycoplasma isolated from the urogenital tract of both men and women. Ureaplasma has 14 known serotypes and is divided into two biovars- Ureaplasma parvum and Ureaplasma urealyticum, they are parasitic organisms that lack a cell wall and are thus extremely sensitive to external factors, including antibiotics. In this study, 30 samples of vaginal swabs were taken by a specialized gynecologist using sterile cotton and speculum swabs from women in the city of Mosul visiting Al-Khansa Teaching Hospital and Al-Batoul Hospital, and other outpatient clinics. Samples were collected from women who use IUDs and have not used antibiotics, from the ages between 25-40 years. PCR was used to detect the presence of Ureaplasma by targeting a specific fragment from the urease gene. The results showed that 13 samples of vaginal swabs gave a positive result indicating a presence ratio of 43% for the presence of Ureaplasma. Our results indicate that the presence of Intrauterine Device (IUD) in women is often accompanied by genital tract infections, depending on personal hygiene. Our results show that molecular detection of Ureaplasma via specific primers in PCR is efficient in the direct detection in vaginal swabs.