iarjcmbIARJCMBIARJCMB2789-6005https://doi.org/10.47310/iarjcmb.2024.v04i01.001Revealing antibiotics analysis against Citrobacter frundii isolated from burn and wound casesAliSalahMahmoudGhadaA. MohammadNineveh Health Department, Al-Jumhuri Teaching Hospital, Mosul, 41001, IraqDepartment of Biology, College of Science, University of Mosul, Mosul City, 41001, IraqOne hundred and twelve swab samples of burn and wounds were collected from patients incoming to the Specialized Center for Burns and Plastic Wounds in Mosul City/ Iraq, from September 2022 to the end of January 2023, for different ages ranging 18 months to 55 years and for both sexes. After culturing 112 wound swabs, traditional biochemical methods and the (API 20E) system for investigation, as well as the Vitek-2 compact system were used to diagnose the bacterial isolates. A molecular diagnostic of Citrobacter species was carried out based on the outcomes of the PCR method by the 16SrRNA gene. This involved sequential analysis of the nitrogenous bases to complete the investigative genes of the bacterial isolates. The results indicated that 5 bacterial isolates of Citrobacter spp which included three isolates belonging to the species C. freundii, and one isolate of both C.werkmanii and C. farmeri. Antimicrobial susceptibility test(AST) of all isolates of Citrobacter spp were carried out by using the Kirby-Bauer’s method showed that all isolates were 100% resistant to antibiotics Doxycycline, Tetracycline and Oxytetracycline whereas the study showed that Amikacin, Trimethoprim, Nalidixic acid Norfloxaine, Levofloxacine and Ciprofloxacin best antibacterial effect against of Citrobacter spp.