srjcmsSRJCMSSRJCMS2788-8851https://doi.org/10.47310/srjcms.2023.v03i02.025A Comparative Study of Leishman Stain and Field’s Stain On Routine Peripheral Smear StudyReshmaJ.SenthilKumarT.YogeeswariP.R.SoniyaGandhiG.Educational and Research Institute, Allied Health Science, Velappanchavadi 600077, Thiruvallur Dist., Tamilnadu, IndiaPeripheral smear study is used to diagnosis the disease condition in blood components, for the peripheral smear stain the most commonly used staining is Romanowsky-type stain they are neutral stain and it is most widely used for the examination of blood from the blood films. Leishman stain is the type of Romanowsky stain, Leishman stain is the mixture of Methylene blue and Eosin dye, it stains the blood cells in violet color, Leishman stain is habitually used stain in hematology. Field’s stain is named after physician John Willian Field, he developed it in 1941. Field’s stain is the combination of Methylene blue, Azure I, Eosin Y dye and the fixative is methanol. Field’s stain is rapid stain used primarily on thin films for malarial parasites, Field’s stain is routinely used in hematology and cytopathologyp. Field’s satin is extremely fast stain. In the present study was comparing the Leishman stain and Field’s stain for this study 60 patient sample is used. Two peripheral smears were prepared from each sample and totally 120 smears, each patients samples were stained in parallel with Leishman stain and Field’s stain. Staining time taken for Leishman stain is 15 minutes and the staining time taken for Field’s stain is 1 minute and the slides were observed under microscope 4, 10, 40 and 100× with oil immersion. The percentage were analyzed statistically. This study shows that Field’ stain better alternative for Leishman stain for routine peripheral smear study and this Field’ stain has advantages in the staining time, clarity view of blood cells and easy to identify the different leukocytes with crisp and clear view of granule of cell and the cytoplasm. Field’ s stain is the better alternative for Leishman stain.