iarjimphIARJIMPHIARJIMPH2709-331Xhttps://doi.org/10.47310/iarjimph.2023.v04i01.013Post laparoscopic cholecystectomy nontuberculous Mycobacterial Surgical site infection: A case seriesVermaRohitGuptaArchitaGuptaVeetheeanveshnaBackground: The rapidly growing nontuberculous mycobacteria (NTM), Mycobacterium fortuitum,and Mycobacterium chelonaehave been reported to cause a range of manifestations ranging from skin and wound infections tosepticemia, meningitis, and endocarditis. Nowadays such cases are seen more frequently sp. SSI due to NTM. This has risen concern among the medical fraternity. Rigid nature of NTM like the production of biofilm and resistance to disinfectants makes them a big reason for worry. There are 4 such cases of post laparoscopic cholecystectomy SSI due to NTM which presented themselves in our hospital. Design and Methods: NTMwas found in four instances of post-surgical wound infections. Ziehl-Neelsen staining, Auramine Rhodaminefluorescence staining, various cultures, and biochemical tests along with CBNAAT and Real-Time PCR, were used to identify thespecies. Results: M. fortuitum, was isolated from three out of four cases whereas andM. chelonaewas isolated from the discharge of one case.NTMisolates evaluated for antibiotic susceptibility pattern were all sensitive to Clarithromycin (100%) and Doxycycline (100%). One case is on treatment with Gentamicin alongside the above-mentioned drugs. Conclusion: NTM is an infection of uncommon nature that can occur after surgical procedures mostly if sterilization is not performed appropriately. Identification ofthese organisms through sensitive techniques and appropriate therapeutic regimen must bedone to curb this recent yet fast-developing menace.