iarjetIARJETIARJET2708-5163https://doi.org/10.47310/iarjet.2021.v02i01.008The Biological and Mechanical Study of Alginate/Honey 3D Printed ScaffoldsSudiptoDattaRanjitBaruaAnkitaDasAmitRoyChowdhuryPallabDattaCentre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah-711103, India.Department of Aerospace Engineering and Applied Mechanics, Indian Institute of Engineering Science and Technology, Shibpur, Howrah-711103, IndiaThe 3D bioprinting technology is getting innovative day to day although this technology is very old. By this technology we can print and fabricate many desired precise biological scaffolds, aligned tissues etc. Various biopolymers like PDMS, alginate, gelatin are being used as the bioinks for printing these scaffolds.  As because of good degradation properties and best biocompatibility alginate scaffolds are commonly being used for this technology. Bioink optimization is also very important like viscosity optimization is necessary because more viscous bioinks will be impossible for extrusion from the nozzle of bioprinted and less viscous bioink will cause undesired structured which cannot be acceptable. After the structure is printed crosslinking of the structure is necessary otherwise the desired structure cannot be obtained. Various crosslinking agents like ZnCl2, CaCl2, and BaCl2 are commonly being used for crosslinking of these printed scaffolds. For improving the cell material interaction blending of various polymers with alginate is necessary. As because honey a natural occurring polymer has wound healing property as well as antioxidant properties can be used with alginate for improving the cell material interaction. In this research work honey is blended with the primary polymer alginate solution and the printed constructs were crosslinked by using 0.5M CaCl2 solution as a printing speed of 50 mm/s. Lower honey concentration is mixed with 5% wt/v alginate solution and were printed. Fibroblast cells were cultured on the printed scaffolds and various characterization like DPPH, MTT and mechanical tensile testing were done.