Histomorphological developmental study of the stomach in the neonatal and adult mice
Enas Shehab Ahmed,* Rabab A. Naser ,Jasim Mohammed Abdullah
The study was done to investigate the histoarchitecture developmental changes that could occur in the stomach of mice between the neonatal and adult periods. To conduct a such project, 4 neonatal mice and 4 adult mice were collected. mice were sacrificed, and measure the weight of the mice and stomach, the organ was dissected and collected, and then fixation with 10 %formalin. In adult local mice the study of stomachs included the description morphologically, histometric measurements, and histological structure by using the light microscope level via stains techniques of Haematoxylinestain and Eosine stain. As a result, the stomach has two curvatures with C-shaped. It is a simple (unilocular) homogenous and smooth Boundary ridge (margo plicatus)without external demarcation between its divisions, a little transparent, and white grayish. The histological examination shows that the stomach wall of the two periods shows the distinctive four compartments of tunics( mucosa, submucosa, muscularis, and serosa). In neonates, the epithelial mucosa showed active mitotic figures (proliferating columnar layer), and the layer of lamina propria consisted of a thin of layer loose connective tissue occupied by simple tubular glands that showed differentiation of not well-demarcated partial and chief cells. The muscularis mucosa of mucosa showed newly differentiating smooth muscle fibers which appear as a very thin layer. The tunic submucosa was a very thin layer composed of fibrous connective tissue and showed mainly fibroblasts. The tunic muscular was differentiated into well-demarcated smooth muscle fibers in the inner which is a circular layer and the outer is the longitudinal layer. The section of the non-glandular part of the stomach in the neonate was composed of four tunica (mucosa, submucosa, muscularis, and serosa). The layer of mucosa showed numerous slightly long mucosal folds lined by non-keratinized stratified squamous epithelial mucosa, the lamina propria- submucosa was composed of a thick layer of fibrous connective tissue and the muscularis mucosa of mucosa was not well demarcated. The tunic muscular was differentiated into well demarcated inner circular layer of smooth muscle fibers and the outer longitudinal layer of smooth muscle fibers. The sections of the glandular part of the stomach in adults displayed a very thick gastric wall composed of an ideal four tunics (mucosa, submucosa, muscularis & serosa). The epithelial mucosa was lined by simple columnar epithelium that was thrown into deep invagination to form gastric pits at the neck region of the gastric gland. The lamina propria was composed of very thick which was occupied by mature simple tubular glands that showed well-demarcated mucous cells, partial cells, and chief cells. The muscularis mucosa of mucosa showed a thick layer of circular and longitudinal smooth muscle fibers, and the tunic submucosa was a very thin layer composed of loose connective tissue. The tunic muscular was differentiated into the well-demarcated inner circular layer of smooth muscle fibers and the outer longitudinal layer of smooth muscle fibers. The non-glandular part of the stomach section the tunica mucosa of adults showed huge mucosal folds lined by keratinized stratified squamous epithelial mucosa, the layer of lamina propria of submucosa was composed of a very thick layer of fibrous connective tissue, and absent muscularis mucosa of mucosa layer. The tunic muscular was differentiated into a well-demarcated
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